Targeted DNA sequencing data for the iSCORE-PD isogenic hESC clone collection, encompassing three complementary analyses: (1) NGS genotyping of 53 mutant clones across 11 PD-linked genes via amplicon sequencing on Illumina iSeq 100 with CRISPResso2 analysis, (2) zygosity testing of a subset of clones in 6 genes via SNP-PCR (NGS or Sanger) to assess zygosity near CRISPR edit sites, and (3) Sanger sequencing of the wild-type allele in 5 DJ1/PARK7 heterozygous clones. ASAP Team: Rio Dataset Name: rio-hesc-targeted-ngs-mutant-zygosity, v1.0 Principal Investigator: Donald Rio Co-Principal Investigator: Frank Soldner; Dirk Hockemeyer; Helen Bateup Contributors: Busquets, Oriol; Li, Hanqin; Syed, Khaja Mohieddin; Alvarez-Jerez, Pilar; Dunnack, Jesse; Lo Bu, Riana; Verma, Yogendra; Pangilinan, Gabriella R.; Martin, Annika; Straub, Jannes; Du, YuXin; Simon, Vivien M.; Poser, Steven; Bush, Zipporiah; Diaz, Jessica; Sahagun, Atehsa; Gao, Jianpu; Hong, Samantha; Hernandez, Dena G.; Levine, Kristin S.; Pochet, Nathalie; Booth, Ezgi O.; Blanchette, Marco; Bateup, Helen S.; Rio, Donald C.; Blauwendraat, Cornelis; Hockemeyer, Dirk; Soldner, Frank Sample Summary: Resources and Additional...
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iSCORE-PD: NGS genotyping and zygosity testing of isogenic hESC clones is published on ASAP CRN Cloud.